The isolation of eugenol and acetyl eugenol from cloves

We have developed a lesson plan for chemistry teachers to conduct an experiment involving the extraction of eugenol from whole cloves by co-distillation with indirect steam, steam that is generated in situ.

isolation of eugenol from cloves lab report discussion

The extract from the done active solvent extraction was test and characterizedvthrough 2 test Bayers test and Ferric chloride FeCl3. Therefore, microwave use consumes 9 kJ per group and the heating mantle kJ per group. The experiment, which is moderately difficult, takes about five hours to do the procedures and analysis.

Steam distillation of clove oil lab report

Dichloromethane can irritate the lungs, skin, and eyes and can affect the central nervous system if breathed so it was used in a fume hood. The organic compound drives into organic layer and transferred to separatory funnel see figure 2 where Indirect steam distillation is a technique used for generating steam in situ, where the water level is kept below the plant material. Samples were analyzed via TLC solvent was mixture of pentane and dichloromethane and characterized by IR spectroscopy. Solvent use was also reduced in two ways, first by optimizing the initial amount required for solid-to-liquid extraction, and second by eliminating the need for subsequent liquid-to-liquid extraction. Experimental Procedure A detailed procedure is provided as Supporting Information. During the process, Eugenol is extracted from the distillate with dichloromethane and analyzed using NMR spectrometers. Clove oil is an irritant and was handled with care. Even compared to recent alternatives developed for extraction of eugenol from cloves, such as use of an espresso machine 8 , microwave extraction is more energy efficient, requires less solvent, and provides comparable yields. Hazards The use of adequate personal protection equipment, including eye protection, by students and instructors was required at all times in the laboratory. References [1] Shan, B. Figure 1. Most essential oils are extracted by direct or indirect steam distillation. The samples were evenly spaced around the microwave carrousel and loaded. The spectra obtained were compared to a reference spectrum to confirm the identity of the product as eugenol

Essential oils are extracted, depending on the nature of the botanical material, by a variety of techniques, including expression cold-presssolvent, enfleurage cold-fat and supercritical fluid SCF extraction, and distillation. After 5 minutes, 2 layers was formed, the bottom aqueous layer was drained and discarded.

References [1] Shan, B. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents.

Clove oil extraction process

References [1] Shan, B. Steam distillation is performing a co-distillation with water where oil can be isolated with lower temperature than the actual boiling point of the two components. The bud also contains a tannin complex, a gum and resin, and a number of glucosides of sterols. Clove oil is an irritant and was handled with care. Samples were analyzed via TLC solvent was mixture of pentane and dichloromethane and characterized by IR spectroscopy. We have developed a lesson plan for chemistry teachers to conduct an experiment involving the extraction of eugenol from whole cloves by co-distillation with indirect steam, steam that is generated in situ. The samples were evenly spaced around the microwave carrousel and loaded. During the process, Eugenol is extracted from the distillate with dichloromethane and analyzed using NMR spectrometers. Indirect steam distillation is a technique used for generating steam in situ, where the water level is kept below the plant material. Therefore, microwave use consumes 9 kJ per group and the heating mantle kJ per group. The experiment, which is moderately difficult, takes about five hours to do the procedures and analysis. Clove oil is reported to have antihistaminic and antispasmotic properties, most likely due to the presence of eugenyl acetate. Steam Distillation with Powdered Cloves and Distillate cloudy After collecting the distillate, the boiling point was recorded. Figure 1.

First part was steam distillation of clove oil. Meanwhile, anhydrous Na2SO4 was added and swirled.

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Experiment 1: Isolation of Eugenol from Cloves by Valerie Ng on Prezi